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Pitchers
- 33 Tanner Andrews
- 44 Sam Armstrong
- 30 Kyle Bischoff
- 4 Darren Bowen
- 38 Kade Bragg
- 41 Ricky Castro
- 45 C. J. Culpepper
- 50 Jack Dougherty
- 4 Ryan Gallagher
- 49 Alejandro Hidalgo
- 15 Hunter Hoopes
- 16 Michael Martinez
- 23 Jaylen Nowlin
- 39 John Stankiewicz
- 19 Logan Whitaker
- 48 Jacob Wosinski
- 35 Gabriel Yanez
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Catchers
Infielders
Outfielders
- 24 Allan Cerda
- 9 Kyle Hess
- 47 Maddux Houghton
- 17 Hendry Mendez
- 26 Kala'i Rosario
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Manager
Coaches
- 55 Andrew Cresci (hitting)
- 24 Yeison Perez (hitting)
- 25 Ryan Ricci (pitching)
- 27 Jesus Sanchez (pitching)
60-day injured list
 7-day injured list
* On Minnesota Twins 40-man roster
~ Development list
# Rehab assignment
∞ Reserve list
‡ Restricted list
§ Suspended list
† Temporarily inactive list
Roster updated September 30, 2025
Transactions
→ More rosters: MiLB • Texas League
→ Minnesota Twins minor league players
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