John Middleton may refer to:

• John Middleton (actor) (born 1953), English actor
• John Middleton (artist), Northern Irish artist, see The Honest Ulsterman
• John Middleton (Norfolk artist) (1827–1856), English artist
• John Powers Middleton (born 1984), American film and television producer
• John Izard Middleton (1785–1849), American archeologist and artist

• John Middleton (c.1373–1441), MP for Northumberland
• John Middleton (MP for City of London), represented City of London (Parliament of England constituency)
• John Middleton (MP for Horsham) (died 1636), English landowner and politician
• John Middleton (1671–1745), Member for Bramber and Horsham
• John Middleton (British Army officer) (1678–1739), Brigadier-General, Member for Aberdeen
• John Myddelton (1685–1747), MP for Denbigh Boroughs 1733–1741 and Denbighshire 1741–1742
• Sir John Middleton (colonial administrator) (1870–1954), British colonial administrator

• John Francis Marchment Middleton (1921–2009), British anthropologist
• John Henry Middleton (1846–1896), British archaeologist and museum director
• John Izard Middleton (1785–1849), American archeologist and artist

• John Middleton (baseball) (1900–1986), American baseball player
• John Middleton (cyclist) (1906–1991), British racing cyclist
• John Middleton (footballer, born 1910) (1910–1971), English football player for Swansea Town, Darlington, Blackpool and Norwich City
• John Middleton (footballer, born 1955), English football player for Bradford City and Macclesfield
• John Middleton (footballer, born 1956) (1956–2016), English football player for Nottingham Forest and Derby County
• John Middleton (cricketer) (1890–1966), English cricketer
• John R. Middleton, American football player and coach
• John Middleton (rugby union), English international rugby union player

• John Middleton (architect) (1820–1885), English architect
• John Middleton (cowboy) (1854–1885), member of the Regulators alongside Billy the Kid during the Lincoln County War
• John Middleton (explorer), travelled to the East Indies with James Lancaster, 1601–1603
• John Middleton (giant) (1578–1623), 2.8m tall man from Hale, England
• John Middleton, 1st Earl of Middleton (1608–1674), general in the Battle of Cropredy Bridge during the English Civil War
• John S. Middleton, American businessman and philanthropist

• John Middleton, a fictional character in American TV series Virgin River

• John Middleton Co., a tobacco manufacturer and subsidiary of Altria

This disambiguation page lists articles associated with the title John Middleton.
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